Immunohistochemical screening of cervical cancer (p16INK4a + Ki-67)
Immunohistochemical testing using dual staining with p16INK4a and Ki-67 markers is used for the early diagnosis of precancerous cervical lesions and cervical cancer.
The method allows the detection of cells with impaired cell cycle regulation characteristic of transformation associated with oncogenic HPV types.
Principle of the method:
- p16INK4a - a marker reflecting cell cycle blockade induced by oncogenic HPV;
- Ki-67 - a marker of cellular proliferative activity.
Simultaneous expression of these markers in the same cell is a sign of neoplastic transformation.
What does the study detect ?
- high-grade squamous intraepithelial lesions (HSIL) and precancerous epithelial changes;
- early stages of cervical cancer;
- cells with a high risk of malignant transformation.