High cancer risk Human Papillomavirus (genotyping 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68), quantification of DNA, indicating the type of virus

To date, scientists have discovered more than 100 species of papillomaviruses. Some of them can cause cancerous diseases, most often uterine cervix. Specific groups according to the degree of risk of developing cancer:

1. The "low risk" group. Genital warts (pointed condyloma) are more often caused. The group includes HPV 6, 11, 40, 42, 43, 44, 54, 61, 70, 72, 81, and CP6108.

2. The "high risk" group. Almost every one of these types was found in uterine cervix cancer, tumors in the anus and in the oral cavity. The group includes 16, 18, 31 and 33, 35, 39, 45, 51, 52, 56, 58, 59, 68, 73 and 82. The listed types are officially recognized as carcinogens.

Thus, the main purpose of the test is to diagnose the risk of developing uterine cervix cancer. The degree of risk depends on the level of contamination – the number of viral particles per 100 thousand cells. The determination of human papillomavirus by polymerase chain reaction (PCR) is characterized by high sensitivity and specificity (98%). The principle of PCR is to identify a unique DNA fragment belonging to a given virus after repeated doubling of the studied genetic material in a test-tube. However, it is worth remembering that the detection of HPV in the material does not yet indicate the presence of cancer in the patient. Quantification of UCC in the material will allow to judge the risk of dysplasia and breast cancer. In the OLYMP CDL branches, PCR analysis are made in REAL-TIME mode - this means that after each hardware cycle (amplification), the amount of DNA in the biomaterial is measured. This procedure reduces the probability of a false positive result to almost zero! The test material is a scraping from the vagina. Therefore, the result of the test indicates the contamination of the human papillomavirus directly at the site of biomaterial collection.